LOCUS       sequence-73695-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Hughes AL, Gottschling DE
  TITLE     An early age increase in vacuolar pH limits mitochondrial function 
            and lifespan in yeast.
  JOURNAL   Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 
            2012 Nov 21.
  PUBMED    23172144
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11654"; journalName: "Nature"; date: "2012-12-13- 
            13"; volume: "492"; issue: "7428"; pages: "261-5"
COMMENT     Sequence Label: pAG306-GPD-ccdB chr I
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..81
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     65..81
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    89..213
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(238..243,244..261,262..268)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(388..407)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aattctactt ttatagttag tctttttttt agttttaaaa caccagaact tagtttcgac
       61 ggattctaga actagtggat cccccatcac aagtttgtac aaaaaagctg aacgagaaac
      121 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
      181 atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt
      241 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt
      301 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
      361 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
      421 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
      481 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
      541 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
      601 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      661 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      721 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      781 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      841 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattc
//
