LOCUS       sequence-73696-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Hughes AL, Gottschling DE
  TITLE     An early age increase in vacuolar pH limits mitochondrial function 
            and lifespan in yeast.
  JOURNAL   Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 
            2012 Nov 21.
  PUBMED    23172144
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11654"; journalName: "Nature"; date: "2012-12-13- 
            13"; volume: "492"; issue: "7428"; pages: "261-5"
COMMENT     Sequence Label: pAG306-GPD-ccdB chr I
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..164
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(205..510)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(307..326)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ggtatcgata agcttgatat cgaattcctg cagcccatca ccactttgta caagaaagct
       61 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
      121 cagactacat aatactgtaa aacacaacat atccagtcac tatggtcgac ctgcagactg
      181 gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt
      241 gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac
      301 actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta
      361 aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg
      421 tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc
      481 tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcagcaaaag
      541 agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc
      601 agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc agaccggaga
      661 tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat
      721 acgctgcttc atagcatacc tctttttgac atacttcggg tatacatatc agtatatatt
      781 cttataccgc aaaaatcagc gcgcaaatac gcatactgtt atctggcttt tagtaagccg
      841 gatccacgcg tttacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaa
//
