LOCUS       sequence-73708-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS4.2 (FF575)
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     290..306
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     290..306
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(340..356)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(341..357)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggctccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tggggccgcc aatatgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtgatcct ctagaactag
      301 tggatccccc gggctgcagg aattcgatat caagcttatc gataccgtcg acctcgaggg
      361 ggggcccggt acccgacgag tcagtaataa acggcgtcaa agtggttgca gccggcacac
      421 acgagtcgtg tttatcaact caaagcacaa atacttttcc tcaacctaaa aataaggcaa
      481 ttagccaaaa acaactttgc gtgtaaacaa cgctcaatac acgtgtcatt ttattattag
      541 ctattgcttc accgccttag ctttctcgtg acctagtcgt cctcgtcttt tcttcttctt
      601 cttctataaa acaataccca aagagctctt cttcttcaca attcagattt caatttctca
      661 aaatcttaaa aactttctct caattctctc taccgtgatc aaggtaaatt tctgtgttcc
      721 ttattctctc aaaatcttcg attttgtttt cgttcgatcc caatttcgta tatgttcttt
      781 ggtttagatt ctgttaatct tagatcgaag acgattttct gggtttgatc gttagatatc
      841 atcttaattc tcgattaggg tttcatagat atcatccgat ttgttcaaat aatttgagtt
      901 ttgtcgaata attactcttc gatttgtgat ttctatctag atctggtg
//
