LOCUS       sequence-73719-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependant (flex) rabies glycoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A
  TITLE     Dissecting local circuits: parvalbumin interneurons underlie broad 
            feedback control of olfactory bulb output.
  JOURNAL   Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027.
            Epub 2013 Nov 14.
  PUBMED    24239125
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.08.027"; journalName: "Neuron"; date: 
            "2013-12-4- 4"; volume: "80"; issue: "5"; pages: "1232-45"
COMMENT     Sequence Label: CAG-Flex-RG
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    82..115
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    186..219
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      226..235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gnnnnactnn ttagatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattat
       61 cgatcggccg catataagct tataacttcg tataatgtat gctatacgaa gttatgggtc
      121 gatggtgaga tctggactag agggtcgatg gtgatgcttg gatcaaaata ggaagaatcc
      181 caatgataac ttcgtataaa gtatcctata cgaagttata ggcgcgccac catggttcct
      241 caggctctcc tgtttgtacc ccttctggtt tttccattgt gttttgggaa attccctatt
      301 tacacgatac cagacaagct tggtccctgg agtccgattg acatacatca cctcagctgc
      361 ccaaacaatt tggtagtgga ggacgaagga tgcaccaacc tgtcagggtt ctcctacatg
      421 gaacttaaag ttggatacat cttagccata aaagtgaacg ggttcacttg cacaggcgtt
      481 gtgacggagg ctgaaaccta cactaacttc gttggttatg tcacaaccac gttcaaaaga
      541 aagcatttcc gcccaacacc agatgcatgt agagccgcgt acaactggaa gatggccggt
      601 gaccccagat atgaagagtc tctacacaat ccgtaccctg actaccgctg gcttcgaact
      661 gtaaaaacca ccaaggagtc tctcgttatc atatctccaa gtgtggcaga tttggaccca
      721 tatgacagat cccttcactc gagggtcttc cctagcggga agtgctcagg agtagcggtg
      781 tcttctacct actgctccac taaccacgat tacaccattt ggatgcccga gaatccgaga
      841 ctagggatgt cttgtgacat ttttaccaat agtagaggga ananagcatc caaagggagt
      901 gagacttgcg gctttgtaga tgaaagaggc ctatatagtc tttnannngc atgcanntca
      961 gtntgtggan ttctaggant nnactatgna tggnnatg
//
