LOCUS       sequence-73720-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependant (flex) rabies glycoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A
  TITLE     Dissecting local circuits: parvalbumin interneurons underlie broad 
            feedback control of olfactory bulb output.
  JOURNAL   Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027.
            Epub 2013 Nov 14.
  PUBMED    24239125
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.08.027"; journalName: "Neuron"; date: 
            "2013-12-4- 4"; volume: "80"; issue: "5"; pages: "1232-45"
COMMENT     Sequence Label: CAG-Flex-RG
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    20..608
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             126..137
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     535..555
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    632..665
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    736..769
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      776..785
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tnnngatctc ngtatcgatg cggggaggcg gcccaaaggg agatccgact cgtctgaggg
       61 cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc
      121 gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca
      181 acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac ggaattgtca
      241 gtgcccaaca gccgaccccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc
      301 gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg
      361 ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca
      421 acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg
      481 aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc
      541 gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaattttt gtaatccaga
      601 ggttgattat cgatcggccg catataagct tataacttcg tataatgtat gctatacgaa
      661 gttatgggtc gatggtgaga tctggactag agggtcgatg gtgatgcttg gatcaaaata
      721 ggaagaatcc caatgataac ttcgtataaa gtatcctata cgaagttata ggcgcgccac
      781 catggctcct cangctctcc tgtttgtacc ccttctggtt tttccattgt gttntngaga
      841 aaattncnct atttacacga taccagacna gctttgnncg cnngnnngta cngattgaca
      901 tacatcanct cagctgnnca aattatttgg gtannggaga ngnnnnatgc accancctgn
      961 cnggntctcn agcatgnnnt nangnngnnn cnt
//
