LOCUS       sequence-73723-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS5.1 (FF572)
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     50..66
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     51..67
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        97..115
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     97..114
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(148..855)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac
       61 ggtatcgata agcttgatat cgaattcctg cagcccattt aggtgacact atagaatatc
      121 actagtaagc tagcttgcat gccggtcctg ctgagcctcg acatgttgtc gcaaaattcg
      181 ccctggaccc gcccaacgat ttgtcgtcac tgtcaaggtt tgacctgcac ttcatttggg
      241 gcccacatac accaaaaaaa tgctgcataa ttctcggggc agcaagtcgg ttacccggcc
      301 gccgtgctgg accgggttga atggtgcccg taactttcgg tagagcggac ggccaatact
      361 caacttcaag gaatctcacc catgcgcgcc ggcggggaac cggagttccc ttcagtgagc
      421 gttattagtt cgccgctcgg tgtgtcgtag atactagccc ctggggcact tttgaaattt
      481 gaataagatt tatgtaatca gtcttttagg tttgaccggt tctgccgctt tttttaaaat
      541 tggatttgta ataataaaac gcaattgttt gttattgtgg cgctctatca tagatgtcgc
      601 tataaaccta ttcagcacaa tatattgttt tcattttaat attgtacata taagtagtag
      661 ggtacaatca gtaaattgaa cggagaatat tattcataaa aatacgatag taacgggtga
      721 tatattcatt agaatgaacc gaaaccggcg gtaaggatct gagctacaca tgctcaggtt
      781 ttttacaacg tgcacaacag aattgaaagc aaatatcatg cgatcatagg cgtctcgcat
      841 atctcattaa agcagggatc ccgcatcaga tctatcaaac acatagattt acgaagatct
      901 ctaacattaa atcatcagag agataagttt agggagcaag ctctttcgct tacacagaga
      961 atcacagagg aaagagactt taagaaaact acaacttttt tgaccactgt gatttctctc
     1021 tgcaagcgaa agtacttaat caccaacaca gta
//
