LOCUS       sequence-73724-e        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS5.1 (FF572)
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(106..112,113..130,131..136))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(161..285)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     291..307
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     291..307
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gacggctcct aactcaaaat ccacacatta tacgagccgg
      121 aagcataaag tgtaaagcct ggggtgccta atggggccgc caatatgact ggatatgttg
      181 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      241 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtgatcc tctagaacta
      301 gtggatcccc caaagaattc ttcttctcac aaataaaccc aaatatatca atctactgtg
      361 ttggtgatta agtactttcg cttgcagaga gaaatcacag tggtcaaaaa agttgtagtt
      421 ttcttaaagt ctctttcctc tgtgattctc tgtgtaagcg aaagagcttg ctccctaaac
      481 ttatctctct gatgatttaa tgttagagat cttcgtaaat ctatgtgttt gatagatctg
      541 atgcgggatc cctgctttaa tgagatatgc gagacgccta tgatcgcatg atatttgctt
      601 tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc
      661 ggtttcggtt cattctaatg aatatatcac ccgttactat cgtattttta tgaataatat
      721 tctccgttca atttactgat tgtaccctac tacttatatg tacaatatta aaatgaaaac
      781 aatatattgt gctgaatagg tttatagcga catctatgat agagcgccac aataacaaac
      841 aattgcgttt tattattaca aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta
      901 aaagactgat tacataaatc ttattcaaat ttcaaaagtg ccccaggggc tagtatctac
      961 gacacaccga gcggcgaact aataacgctc actgaaggga actccggttc cccgccggcg
     1021 cgcatgggtg agattccttg aagttgagta t
//
