LOCUS       sequence-73728-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AmCyan while the mCherry gene has been mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  TITLE     AmCyan-P2A-Xerry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AmCyan-P2A-Xerry
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..72
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             100..120
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             136..822
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPHAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKMTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHAVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPF"
     CDS             844..900
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 accgtcagat cccgtctccg tacgatggcc acaaccatgg tgcacgtgga tccaaaaaag
       61 aagagaaagg tagatccaaa aaagaagaga aaggtagatc caaaaaagaa gagaaaggta
      121 cacgtgtcca cgcgtatggc tctttcaaac aagtttatcg gagatgacat gaaaatgacc
      181 taccatatgg atggctgtgt caatgggcat tactttaccg tcaaaggtga aggcagcggg
      241 aagccatacg aagggacgca gacctcgact tttaaagtca ccatggccaa cggtgggccc
      301 catgcattct cctttgacat actatctaca gtgttcatgt atggaaatcg atgctttact
      361 gcgtatccta ccagtatgcc cgactatttc aaacaagcat ttcctgacgg aatgtcatat
      421 gaaaggactt ttacctatga agatggagga gttgctacag ccagttggga aataagcctt
      481 aaaggcaact gctttgagca caaatccacg tttcatggag tgaactttcc tgctgatgga
      541 cctgtgatgg cgaagatgac aactggttgg gacccatctt ttgagaaaat gactgtctgc
      601 gatggaatat tgaagggtga tgtcaccgcg ttcctcatgc tgcaaggagg tggcaattac
      661 agatgccaat tccacacttc ttacaagaca aaaaaaccgg tgacgatgcc accaaaccat
      721 gcggtggaac atcgcattgc gaggaccgac cttgacaaag gtggcaacag tgttcagctg
      781 acggagcacg ctgttgcaca tataacctct gttgtccctt tcgcgcgcga gacgggaagc
      841 ggagctacta acttcagcct gctgaagcag gctggcgacg tggaggagaa ccctggacct
      901 ggtctccggc cgatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      961 atgcgctt
//
