LOCUS       sequence-73735-e        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS5.2 (FF576)
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(106..112,113..130,131..136))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(161..285)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     291..307
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     291..307
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gacggctcct aactcaaaat ccacacatta tacgagccgg
      121 aagcataaag tgtaaagcct ggggtgccta atggggccgc caatatgact ggatatgttg
      181 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      241 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtgatcc tctagaacta
      301 gtggatcccc caagaattct tcttctcaca aataaaccca aatatatcaa tctactgtgt
      361 tggtgattaa gtactttcgc ttgcagagag aaatcacagt ggtcaaaaaa gttgtagttt
      421 tcttaaagtc tctttcctct gtgattctct gtgtaagcga aagagcttgc tccctaaact
      481 tatctctctg atgatttaat gttagagatc ttcgtaaatc tatgtgtttg atagatctga
      541 tgcgggatcc ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt
      601 caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg
      661 gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat gaataatatt
      721 ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa aatgaaaaca
      781 atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca ataacaaaca
      841 attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa
      901 aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct agtatctacg
      961 acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc ccgccggcgc
     1021 gcatgggtga gattccttga agttgagtat tg
//
