LOCUS       sequence-73738-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows co-translational incorporation of the fluorescent ncAA Anap 
            directly into the target protein; expresses amber suppressor tRNA 
            and the synthetase AnapRS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Chatterjee A, Guo J, Lee HS, Schultz PG
  TITLE     A genetically encoded fluorescent probe in Mammalian cells.
  JOURNAL   J Am Chem Soc. 2013 Aug 28;135(34):12540-3. doi: 10.1021/ja4059553. 
            Epub 2013 Aug 14.
  PUBMED    23924161
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja4059553";
            journalName: "J Am Chem Soc"; date: "2013-08-28- 28"; volume: "135";
            issue: "34"; pages: "12540-3"
COMMENT     Sequence Label: pANAP
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        114..208
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    162..180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    190..208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            join(213..246,247..249,250..296)
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        310..404
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    358..376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    386..404
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            join(409..442,443..445,446..492)
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        506..600
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    554..572
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    582..600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            join(605..638,639..641,642..688)
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        702..796
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    750..768
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    778..796
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            join(801..834,835..837,838..884)
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
ORIGIN
        1 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
       61 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctaatatttg
      121 catgtcgcta tgtgttctgg gaaatcacca taaacgtgaa atccctatca gtgatagaga
      181 cttataagtt ccctatcagt gatagagaca ccgcccggat ggtggaatcg gtagacacaa
      241 gggattctaa atccctcggc gttcgcgctg tgcgggttca agtcccgctc cgggtatttt
      301 ttggatctaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatcc
      361 ctatcagtga tagagactta taagttccct atcagtgata gagacaccgc ccggatggtg
      421 gaatcggtag acacaaggga ttctaaatcc ctcggcgttc gcgctgtgcg ggttcaagtc
      481 ccgctccggg tattttttgg atctaatatt tgcatgtcgc tatgtgttct gggaaatcac
      541 cataaacgtg aaatccctat cagtgataga gacttataag ttccctatca gtgatagaga
      601 caccgcccgg atggtggaat cggtagacac aagggattct aaatccctcg gcgttcgcgc
      661 tgtgcgggtt caagtcccgc tccgggtatt ttttggatct aatatttgca tgtcgctatg
      721 tgttctggga aatcaccata aacgtgaaat ccctatcagt gatagagact tataagttcc
      781 ctatcagtga tagagacacc gcccggatgg tggaatcggt agacacaagg gattctaaat
      841 ccctcggcgt tcgcgctgtg cgggttcaag tcccgctccg ggtatttttg ggatctaata
      901 tttgcatgtc gctatgtgtt ctgggaaatc accataaacg tgaa
//
