LOCUS       sequence-73739-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows co-translational incorporation of the fluorescent ncAA Anap 
            directly into the target protein; expresses amber suppressor tRNA 
            and the synthetase AnapRS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Chatterjee A, Guo J, Lee HS, Schultz PG
  TITLE     A genetically encoded fluorescent probe in Mammalian cells.
  JOURNAL   J Am Chem Soc. 2013 Aug 28;135(34):12540-3. doi: 10.1021/ja4059553. 
            Epub 2013 Aug 14.
  PUBMED    23924161
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja4059553";
            journalName: "J Am Chem Soc"; date: "2013-08-28- 28"; volume: "135";
            issue: "34"; pages: "12540-3"
COMMENT     Sequence Label: pANAP
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca tggaagagca
      121 ataccgcccg gaagagatag aatccaaagt acagcttcat tgggatgaga agcgcacatt
      181 tgaagtaacc gaagacgaga gcaaagagaa gtattactgc ttttctggcc ctccctatcc
      241 ttctggtcga ctacacatgg gccacgtacg taactacacc atcggtgacg tgatcgcccg
      301 ctaccagcgt atgctgggca aaaacgtcct gcagccgatc ggctgggacg cgtttggtct
      361 gcctgcggaa ggcgcggcgg tgaaaaacaa caccgctccg gcaccgtgga cgtacgacaa
      421 catcgcgtat atgaaaaacc agctcaaaat gctgggcttt ggttatgact ggagccgcga
      481 gctggcaacc tgtacgccgg aatactaccg ttgggaacag aaattcttca ccgagctgta
      541 taaaaaaggc ctggtatata agaagacttc tgcggtcaac tggtgtccga acgaccagac
      601 cgtactggcg aacgaacaag ttatcgacgg ctgctgctgg cgctgcgata ccaaagttga
      661 acgtaaagag atcccgcagt ggtttatcaa aatcactgct tacgctgacg agctgctcaa
      721 cgatctggat aaactggatc actggcctga caccgttaaa accatgcagc gtaactggat
      781 cggtcgttcc gaaggcgtgg agatcacctt caacgttaac gactatgaca acacgctgac
      841 cgtttacact acccgcccgg acacctttat gggttgtacc tacctggcgg tagctgcggg
      901 tcatccgctg gcg
//
