LOCUS       sequence-73748-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Kaper T, Lager I, Looger LL, Chermak D, Frommer WB
  TITLE     Fluorescence Resonance Energy Transfer sensors for quantitative 
            monitoring of pentose and disaccharide accumulation in bacteria.
  JOURNAL   Biotechnol Biofuels. 2008 Jun 3. 1(1):11.
  PUBMED    18522753
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels. 2008 Jun 3. 1(1):11."
COMMENT     Sequence Label: pGWF1
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(20..733,734..736))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(65..86)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     414..433
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     673..694
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    744..768
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcagccgga atcaagcttt tacttgtaca gctcgtccat gccgagagtg atcccggcgg
       61 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
      121 cggactggta gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
      181 tgttctgctg gtagtggtcg gcgagctgca cgccgccgtc ctcgatgttg tggcggatct
      241 tgaagttggc cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtggctgt
      301 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
      361 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
      421 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
      481 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac tgcaggccgt
      541 agcccagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatca
      601 gcttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
      661 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
      721 cctcgccctt gctcaccgat atcaccactt tgtacaagaa agctgaacga gaaacgtaaa
      781 atgatataaa tatcaatata
//
