LOCUS       sequence-73754-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For knock-in donor vector by CRISPR/CAS system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R
  TITLE     One-Step Generation of Mice Carrying Reporter and Conditional 
            Alleles by CRISPR/Cas-Mediated Genome Engineering.
  JOURNAL   Cell. 2013 Aug 27. pii: S0092-8674(13)01016-7. doi: 
            10.1016/j.cell.2013.08.022.
  PUBMED    23992847
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Aug 27. pii: S0092-8674(13)01016-7. doi: 
            10.1016/j.cell.2013.08.022."
COMMENT     Sequence Label: Nanog-2A-mCherry
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(102..158)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
       61 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca ttggcccggg attctcttcg
      121 acatcccctg cttgtttcaa cagggagaag ttagtggcgc tagctatttc acctggtgga
      181 gtcacagagt agttcaggaa taattccaag gcttgtgggg tgctaaaatg cgcatggctt
      241 tccctagtgg cttccaaatt cacctccaaa tcactggcag agaagttttg ctgcaactgt
      301 acgtaaggct gcagaaagtc ctccccgaag ttatggagcg gagcagcatt ccaaggctgg
      361 ccgttccagg actgagcggt ccaggcctgg ctgctccaag ttgggttggt ccaggtctgg
      421 ttgttccaag ttgggttggt ccaggtctgg ctgctccaag ttgggttggt ccaagtctgg
      481 ctgccccaca tggaaaggct tccagatgcg ttcaccagat agccctgggg atagctgcaa
      541 tggatgctgg gatactccac tggtgctgag cccttctgaa agaggtgatt aatgcaatga
      601 gtgaaaaggg gactctaaag atgtaaactg cagaaatact gaaaagatta gcatggccgc
      661 acaggccgtt aaccccagca ctcctgagac taggcaggca gatctctgac tgccagggct
      721 acatagagag agcctgcctc aaagaaccaa agatcacaag aaagagtgcg tg
//
