LOCUS       sequence-73779-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector of N-terminal His-tagged PercevalHR, a 
            fluorescent sensor of ATP-to-ADP ratio..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Tantama M, Martinez-Francois JR, Mongeon R, Yellen G
  TITLE     Imaging energy status in live cells with a fluorescent biosensor of 
            the intracellular ATP-to-ADP ratio.
  JOURNAL   Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550.
  PUBMED    24096541
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2550. doi"; pages: " 
            10.1038/ncomms3550"
COMMENT     Sequence Label: pRsetB-PercevalHR
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(295..546)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(337..358)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(748..768)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     RBS             782..804
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(835..854)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(836..854)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 ggcgaagcat tggagaccgt agcccagggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatca gcttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccatg cctccggaac cgcccttgta
      301 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      361 atcgcgcttc tcgttggggt ctttgctcag cttggactgg aagctcaggt agtggttgtc
      421 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      481 cacgccgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      541 cttgtcggcg gtgatgtaga cgttgtcgct gttgtagcct gcagagtaca cccttcctcg
      601 gtacctctcg aagatgccgc cctggacgcc agagcctttg acttcagaga cggtcatacc
      661 cacatatcca gcgtccgaga gagccttctt aacgatctcc agcttttcgg gcctgatgat
      721 ggattccacc tttttcatgc tagctccatg gtgatggtga tggtgatgtt tcatatgtat
      781 atctccttct taaagttaaa caaaattatt tctagaggga aaccgttgtg gtctccctat
      841 agtgagtcgt attaatttcg cgggatcgag atcctgcatt aatgaatcgg ccaacgcgcg
      901 g
//
