LOCUS       sequence-73781-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector of N-terminal His-tagged PercevalHR, a 
            fluorescent sensor of ATP-to-ADP ratio..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Tantama M, Martinez-Francois JR, Mongeon R, Yellen G
  TITLE     Imaging energy status in live cells with a fluorescent biosensor of 
            the intracellular ATP-to-ADP ratio.
  JOURNAL   Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550.
  PUBMED    24096541
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2550. doi"; pages: " 
            10.1038/ncomms3550"
COMMENT     Sequence Label: pRsetB-PercevalHR
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(83..104)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(344..363)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cggcatggac gagctgtaca agggcggttc cggaggcatg gtgagcaagg gcgaggagct
       61 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat
      181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
      241 tctccaatgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      421 catcgacttc aaggaggacg gcaacatcct ggggcacaag cttgagtaca acggcaccat
      481 agtagatctg atccctaagg taaaaattga actagtggtg aaggaggagg acgtcgataa
      541 cgtgatagac attatttgcg aaaatgcccg cacaggtaac cccggcgacg gtaagatatt
      601 tgtgatccca gtggagcgag tggtcagggt gcgaaccaaa gaggagggag catctggtgg
      661 tggatccggt ggtggcggtg catctggtat gaaaaaggtt gaagctatta ttcgcccaga
      721 gaaactggag attgttaaga aggccctgtc cgacgccggc tatgttggca tgaccgtttc
      781 cgaggttaaa ggccgcggtg ccggcggtgg cgacctgatt cctaaggtga agatcgagct
      841 ggttgttaag gaggaagacg ttgataatgt tatcgacatt atctgcgaga acgcccgcac
      901 tggcaatcct ggtgacggca aaatcttcgt tataccggtc gagcgaattg tgc
//
