LOCUS       sequence-73803-e        3678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative feedback plasmid from oscillator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J
  TITLE     A sensing array of radically coupled genetic 'biopixels'.
  JOURNAL   Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
  PUBMED    22178928
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-12-18- 18"; volume: "481"; issue: "7379"; 
            pages: "39-44"
COMMENT     Sequence Label: pTD103aiiA(Cm)
FEATURES             Location/Qualifiers
     source          1..3678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             968..979
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1789..1875
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1910..1927)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2044..2589)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2124..2143)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2703..2797)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2818..3477)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3392..3411
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3478..3580)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3646..3664
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagttaa tttttaaagt atgggcaatc aattgctcct gttaaaattg ctttagaaat
       61 actttggcag cggtttgttg tattgagttt catttgcgca ttggttaaat ggaaagtgac
      121 agtacgctca ctgcagccta atatttttga aatatcccaa gagctttttc cttcgcatgc
      181 ccacgctaaa cattcttttt ctcttttggt taaatcgttg tttgatttat tatttgctat
      241 atttattttt cgataattat caactagaga aggaacaatt aatggtatgt tcatacacgc
      301 atgtaaaaat aaactatcta tatagttgtc tttttctgaa tgtgcaaaac taagcattcc
      361 gaagccattg ttagccgtat gaatagggaa actaaaccca gtgataagac ctgatgtttt
      421 cgcttcttta attacatttg gagatttttt atttacagca ttgttttcaa atatattcca
      481 attaattggt gaatgattgg agttagaata atctactata ggatcatatt ttattaaatt
      541 agcgtcatca taatattgcc tccatttttt agggtaatta tctagaattg aaatatcaga
      601 tttaaccata gaatgaggat aaatgatcgc gagtaaataa tattcacaat gtaccatttt
      661 agtcatatca gataagcatt gattaatatc attattgctt ctacaagctt taattttatt
      721 aattattctg tatgtgtcgt cggcatttat gtttttcata cccatctctt tatccttacc
      781 tattgtttgt cgcaagtttt gcgtgttata tatcattaaa acggtaatgg attgacattt
      841 gattctaata aattggattt ttgtcacact attgtatcgc tgggaataca attacttaac
      901 ataagcacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaatga
      961 attcattaaa gaggagaaag gtaccatgac agtaaagaaa ctttatttca tcccagcagg
     1021 tcgttgcatg ttggatcatt cgtctgttaa cagtgcgtta acaccgggga aactattaaa
     1081 cttgccggtg tggtgttatc ttttggagac ggaagaaggt cctattttag tagacacagg
     1141 tatgccagaa agtgcagtta ataatgaagg gctttttaac ggtacatttg ttgaaggaca
     1201 gatcttaccg aaaatgactg aagaagatag aatcgtgaat atattaaagc gtgtagggta
     1261 tgagccggac gaccttttat atattattag ttctcactta cattttgatc atgcaggagg
     1321 aaacggtgct tttacaaata caccaattat tgtgcagcga acggaatatg aggcagcact
     1381 tcatagagaa gaatatatga aagaatgtat attaccgcat ttgaactaca aaattattga
     1441 aggggattat gaagtggtac caggtgttca attattgtat acgccaggtc attctccagg
     1501 ccatcagtcg ctattcattg agacggagca atccggttca gttttattaa cgattgatgc
     1561 atcgtacacg aaagagaatt ttgaagatga agtgccgttc gcaggatttg atccagaatt
     1621 agctttatct tcaattaaac gtttaaaaga agttgtgaaa aaagagaaac caattatttt
     1681 ctttggtcat gatatagagc aggaaaagag ttgtagagtg ttcccggaat atatagcagc
     1741 gaacgacgaa aattacgccc ttgcagcgta aacgcgtgct agaggcatca aataaaacga
     1801 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1861 ctgagtagga caaatccgcc gccctagacc tagggatata ttccgcttcc tcgctcactg
     1921 actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag
     1981 atttcctgga agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc
     2041 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     2101 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt
     2161 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     2221 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
     2281 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2341 ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag
     2401 gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac
     2461 tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa
     2521 aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa
     2581 cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt
     2641 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgtt actagtgctt
     2701 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     2761 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     2821 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     2881 gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     2941 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     3001 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     3061 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     3121 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     3181 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     3241 gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat
     3301 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     3361 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     3421 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     3481 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     3541 ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccagat
     3601 atcgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     3661 aggccctttc gtcttcac
//
