LOCUS       sequence-73809-e        5109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lux-regulated expression of sodA enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J
  TITLE     A sensing array of radically coupled genetic 'biopixels'.
  JOURNAL   Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
  PUBMED    22178928
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-12-18- 18"; volume: "481"; issue: "7379"; 
            pages: "39-44"
COMMENT     Sequence Label: pZSm45 sodA
FEATURES             Location/Qualifiers
     source          1..5109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             962..973
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1651..1737
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      2236..2458
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2506..3456
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3987..4081)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4093..4884)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttaattttta aagtatgggc aatcaattgc tcctgttaaa attgctttag aaatactttg
       61 gcagcggttt gttgtattga gtttcatttg cgcattggtt aaatggaaag tgacagtacg
      121 ctcactgcag cctaatattt ttgaaatatc ccaagagctt tttccttcgc atgcccacgc
      181 taaacattct ttttctcttt tggttaaatc gttgtttgat ttattatttg ctatatttat
      241 ttttcgataa ttatcaacta gagaaggaac aattaatggt atgttcatac acgcatgtaa
      301 aaataaacta tctatatagt tgtctttttc tgaatgtgca aaactaagca ttccgaagcc
      361 attgttagcc gtatgaatag ggaaactaaa cccagtgata agacctgatg ttttcgcttc
      421 tttaattaca tttggagatt ttttatttac agcattgttt tcaaatatat tccaattaat
      481 tggtgaatga ttggagttag aataatctac tataggatca tattttatta aattagcgtc
      541 atcataatat tgcctccatt ttttagggta attatctaga attgaaatat cagatttaac
      601 catagaatga ggataaatga tcgcgagtaa ataatattca caatgtacca ttttagtcat
      661 atcagataag cattgattaa tatcattatt gcttctacaa gctttaattt tattaattat
      721 tctgtatgtg tcgtcggcat ttatgttttt catacccatc tctttatcct tacctattgt
      781 ttgtcgcaag ttttgcgtgt tatatatcat taaaacggta atggattgac atttgattct
      841 aataaattgg atttttgtca cactattgta tcgctgggaa tacaattact taacataagc
      901 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atgaattcat
      961 taaagaggag aaaggtacca tgagctatac cctgccatcc ctgccgtatg cttacgatgc
     1021 cctggaaccg cacttcgata agcagaccat ggaaatccac cacaccaaac accatcagac
     1081 ctacgtaaac aacgccaacg cggcgctgga aagcctgcca gaatttgcca acctgccggt
     1141 tgaagagctg atcaccaaac tggaccagct gccagcagac aagaaaaccg tactgcgcaa
     1201 caacgctggc ggtcacgcta accacagcct gttctggaaa ggtctgaaaa aaggcaccac
     1261 cctgcagggt gacctgaaag cggctatcga acgtgacttc ggctccgttg ataacttcaa
     1321 agcagaattt gaaaaagcgg cagcttcccg ctttggttcc ggctgggcat ggctggtgct
     1381 gaaaggcgat aaactggcgg tggtttctac tgctaaccag gattctccgc tgatgggtga
     1441 agctatttct ggcgcttccg gcttcccgat tatgggcctg gatgtgtggg aacatgctta
     1501 ctacctgaaa ttccagaacc gccgtccgga ctacattaaa gagttctgga acgtggtgaa
     1561 ctgggacgaa gcagcggcac gttttgcggc gaaaaaagca gcgaacgacg aaaattacgc
     1621 ccttgcagcg taaacgcgtg ctagaggcat caaataaaac gaaaggctca gtcgaaagac
     1681 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     1741 ccgccctaga cctagggaca gtaagacggg taagcctgtt gatgataccg ctgccttact
     1801 gggtgcatta gccagtctga atgacctgtc acgggataat ccgaagtggt cagactggaa
     1861 aatcagaggg caggaactgc tgaacagcaa aaagtcagat agcaccacat agcagacccg
     1921 ccataaaacg ccctgagaag cccgtgacgg gcttttcttg tattatgggt agtttccttg
     1981 catgaatcca taaaaggcgc ctgtagtgcc atttaccccc attcactgcc agagccgtga
     2041 gcgcagcgaa ctgaatgtca cgaaaaagac agcgactcag gtgcctgatg gtcggagaca
     2101 aaaggaatat tcagcgattt gcccgagctt gcgagggtgc tacttaagcc tttagggttt
     2161 taaggtctgt tttgtagagg agcaaacagc gtttgcgaca tccttttgta atactgcgga
     2221 actgactaaa gtagtgagtt atacacaggg ctgggatcta ttctttttat ctttttttat
     2281 tctttcttta ttctataaat tataaccact tgaatataaa caaaaaaaac acacaaaggt
     2341 ctagcggaat ttacagaggg tctagcagaa tttacaagtt ttccagcaaa ggtctagcag
     2401 aatttacaga tacccacaac tcaaaggaaa aggacaagta attatcattg actagcccat
     2461 ctcaattggt atagtgatta aaatcaccta gaccaattga gatgtatgtc tgaattagtt
     2521 gttttcaaag caaatgaact agcgattagt cgctatgact taacggagca tgaaaccaag
     2581 ctaattttat gctgtgtggc actactcaac cccacgattg aaaaccctac aaggaaagaa
     2641 cggacggtat cgttcactta taaccaatac gctcagatga tgaacatcag tagggaaaat
     2701 gcttatggtg tattagctaa agcaaccaga gagctgatga cgagaactgt ggaaatcagg
     2761 aatcctttgg ttaaaggctt taagattttc cagtggacaa actatgccaa gttctcaagc
     2821 gaaaaattag aattagtttt tagtgaagag atattgcctt atcttttcca gttaaaaaaa
     2881 ttcataaaat ataatctgga acatgttaag tcttttgaaa acaaatactc tatgaggatt
     2941 tatgagtggt tattaaaaga actaacacaa aagaaaactc acaaggcaaa tatagagatt
     3001 agccttgatg aatttaagtt catgttaatg cttgaaaata actaccatga gtttaaaagg
     3061 cttaaccaat gggttttgaa accaataagt aaagatttaa acacttacag caatatgaaa
     3121 ttggtggttg ataagcgagg ccgcccgact gatacgttga ttttccaagt tgaactagat
     3181 agacaaatgg atctcgtaac cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa
     3241 ataccaacaa ccattacatc agattcctac ctacgtaacg gactaagaaa aacactacac
     3301 gatgctttaa ctgcaaaaat tcagctcacc agttttgagg caaaattttt gagtgacatg
     3361 caaagtaagc atgatctcaa tggttcgttc tcatggctca cgcaaaaaca acgaaccaca
     3421 ctagagaaca tactggctaa atacggaagg atctgaggtt cttatggctc ttgtatctat
     3481 cagtgaagca tcaagactaa caaacaaaag tagaacaact gttcaccgtt agatatcaaa
     3541 gggaaaactg tccatatgca cagatgaaaa cggtgtaaaa aagatagata catcagagct
     3601 tttacgagtt tttggtgcat ttaaagctgt tcaccatgaa cagatcgaca atgtaacaga
     3661 tgaacagcat gtaacaccta atagaacagg tgaaaccagt aaaacaaagc aactagaaca
     3721 tgaaattgaa cacctgagac aacttgttac agctcaacag tcacacatag acagcctgaa
     3781 acaggcgatg ctgcttatcg aatcaaagct gccgacaaca cgggagccag tgacgcctcc
     3841 cgtggggaaa aaatcatggc aattctggaa gaaatagcgc tttcagccgg caaaccggct
     3901 gaagccggat ctgcgattct gataacaaac tagcaacacc agaacagccc gtttgcgggc
     3961 agcaaaaccc gtacactagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     4021 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     4081 ccaagcgagc tcttatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
     4141 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
     4201 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
     4261 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
     4321 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
     4381 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
     4441 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
     4501 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
     4561 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
     4621 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
     4681 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
     4741 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
     4801 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
     4861 ttcggcgatc accgcttccc tcatgatgtt taactttgtt ttagggcgac tgccctgctg
     4921 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
     4981 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac
     5041 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
     5101 catgacgtc
//
