LOCUS       sequence-73811-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Wu S, Ying G, Wu Q, Capecchi MR
  TITLE     A protocol for constructing gene targeting vectors: generating 
            knockout mice for the cadherin family and beyond.
  JOURNAL   Nat Protoc. 2008  . 3(6):1056-76.
  PUBMED    18546598
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2008  . 3(6):1056-76."
COMMENT     Sequence Label: pWS-TK6
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(132..256)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        501..563
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           585..624
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
ORIGIN
        1 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
      181 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
      241 tcttgtacaa agtggttgat tcgaggctgc taacaaatcg agcagtgtgg ttttcaagag
      301 gaagcaaaaa gcctctccac ccaggcctgg aatgtttcca cccaatgtcg agcagtgtgg
      361 ttttgcaaga ggaagcaaaa agcctctcca cccaggcctg gaatgtttcc acccaatgtc
      421 gagcaaaccc cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg
      481 cggcgcggtc cgaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag
      541 cgaccctgca gcgacccgct taacagcgtc aacagcgtgc cgcagatctt ggtggcgtga
      601 aactcccgca cctcttcggc cagcgccttg tagaagcgcg tatggcttcg taccccggcc
      661 atcaacacgc gtctgcgttc gaccaggctg cgcgttctcg cggccatagc aaccgacgta
      721 cggcgttgcg ccctcgccgg cagcaagaag ccacggaagt ccgcccggag cagaaaatgc
      781 ccacgctact gcgggtttat atagacggtc cccacgggat ggggaaaacc accaccacgc
      841 aactgctggt ggccctgggt tcgcgcgacg atatcgtcta cgtacccgag ccgatgactt
      901 actggcgggt gctgggggct tccgagacaa tc
//
