LOCUS       sequence-73812-e        4695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ArsR-regulated expression of luxI-laa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J
  TITLE     A sensing array of radically coupled genetic 'biopixels'.
  JOURNAL   Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
  PUBMED    22178928
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-12-18- 18"; volume: "481"; issue: "7379"; 
            pages: "39-44"
COMMENT     Sequence Label: pZS47a luxI-laa RBS
FEATURES             Location/Qualifiers
     source          1..4695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             590..601
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1240..1326
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1861..2811)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2859..3081
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3573..3667)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3679..4470)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tttccaagtt atctcaccta ccttaaggta atagtgtgat taatcatatg cgtttttggt
       61 tatgtgttgt ttgacttaat atcagagccg agagatactt gttttctaca aaggagaggg
      121 aaatgttgca actaacacca cttcagttat ttaaaaacct gtccgatgaa acccgtttgg
      181 gtatcgtgtt gttgctcagg gagatgggag agttgtgcgt gtgtgatctt tgcatggcac
      241 tggatcaatc acagcccaaa atatcccgtc atctggcgat gctacgggaa agtggaatcc
      301 ttctggatcg taaacaggga aaatgggttc actaccgctt atcaccgcat attccttcat
      361 gggctgccca gattattgag caggcctggt taagccaaca ggacgacgtt caggtcatcg
      421 cacgcaagct ggcttcagtt aactgctccg gtagcagtaa ggctgtctgc atctaaaaaa
      481 tttgcctgaa catatatgtt ttatcaaatg cgaggtattt aagatgaaaa cgttaatggt
      541 atttgacccg gcgatgtgtt gcagcaccgg tatcactaga gaattcatta aagaggagaa
      601 aggtaccatg actataatga taaaaaaatc ggattttttg gcaattccat cggaggagta
      661 taaaggtatt ctaagtcttc gttatcaagt gtttaagcaa agacttgagt gggacttagt
      721 tgtagaaaat aaccttgaat cagatgagta tgataactca aatgcagaat atatttatgc
      781 ttgtgatgat actgaaaatg taagtggatg ctggcgttta ttacctacaa caggtgatta
      841 tatgctgaaa agtgtttttc ctgaattgct tggtcaacag agtgctccca aagatcctaa
      901 tatagtcgaa ttaagtcgtt ttgctgtagg taaaaatagc tcaaagataa ataactctgc
      961 tagtgaaatt acaatgaaac tatttgaagc tatatataaa cacgctgtta gtcaaggtat
     1021 tacagaatat gtaacagtaa catcaacagc aatagagcga tttttaaagc gtattaaagt
     1081 tccttgtcat cgtattggag acaaagaaat tcatgtatta ggtgatacta aatcggttgt
     1141 attgtctatg cctattaatg aacagtttaa aaaagcagtc ttaaatgcag cgaacgacga
     1201 aaattacgcc cttgcagcgt aaacgcgtgc tagaggcatc aaataaaacg aaaggctcag
     1261 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     1321 acaaatccgc cgccctagac ctagggtacg ggttttgctg cccgcaaacg ggctgttctg
     1381 gtgttgctag tttgttatca gaatcgcaga tccggcttca ggtttgccgg ctgaaagcgc
     1441 tatttcttcc agaattgcca tgattttttc cccacgggag gcgtcactgg ctcccgtgtt
     1501 gtcggcagct ttgattcgat aagcagcatc gcctgtttca ggctgtctat gtgtgactgt
     1561 tgagctgtaa caagttgtct caggtgttca atttcatgtt ctagttgctt tgttttactg
     1621 gtttcacctg ttctattagg tgttacatgc tgttcatctg ttacattgtc gatctgttca
     1681 tggtgaacag ctttaaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt
     1741 acaccgtttt catctgtgca tatggacagt tttccctttg atatctaacg gtgaacagtt
     1801 gttctacttt tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc
     1861 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
     1921 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
     1981 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
     2041 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
     2101 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
     2161 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
     2221 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
     2281 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
     2341 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
     2401 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
     2461 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
     2521 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
     2581 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
     2641 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
     2701 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
     2761 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
     2821 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     2881 agtccttttc ccgggagatc tgggtatctg taaattctgc tagacctttg ctggaaaact
     2941 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     3001 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     3061 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     3121 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     3181 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     3241 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     3301 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     3361 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     3421 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     3481 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     3541 ggcttacccg tcttactgtc cctagtgctt ggattctcac caataaaaaa cgcccggcgg
     3601 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     3661 aggagtccaa gcgagctctt atttgccgac taccttggtg atctcgcctt tcacgtagtg
     3721 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
     3781 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
     3841 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
     3901 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
     3961 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
     4021 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
     4081 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
     4141 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
     4201 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
     4261 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
     4321 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
     4381 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
     4441 cgatacttcg gcgatcaccg cttccctcat gatgtttaac tttgttttag ggcgactgcc
     4501 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     4561 ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat
     4621 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
     4681 tgagcgcatg acgtc
//
