LOCUS       sequence-73817-e        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Wu S, Ying G, Wu Q, Capecchi MR
  TITLE     A protocol for constructing gene targeting vectors: generating 
            knockout mice for the cadherin family and beyond.
  JOURNAL   Nat Protoc. 2008  . 3(6):1056-76.
  PUBMED    18546598
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2008  . 3(6):1056-76."
COMMENT     Sequence Label: pWS-TK2
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(106..112,113..130,131..136))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(161..285)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gacggatcct aactcaaaat ccacacatta tacgagccgg
      121 aagcataaag tgtaaagcct ggggtgccta atgcggccgc catagtgact ggatatgttg
      181 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      241 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgttgatt cgaggtgatg
      301 gtgacgatga attcgagctc ggtacccggg gatcttccgg gcaggctcgc gttccgaggc
      361 cggccgagca gataggtctt tgggatgtaa agcagctgcc cggggtcccg aggaaactcg
      421 gccgtggtga ccaacacgaa acaaaagcgc tcggcgtacc accgaagcat gggcacggat
      481 gccgtagtca ggttgagttc gcccgggggc gccaagcgtc cgcgctgggg gtcgctggcg
      541 tcgggggtgt tgggcaacca cagacgcccg gtgtttgtgt cgcgccagta cgtgcgggcc
      601 aaccccagac cgtgcaaaaa ccacgggtcg atttgctccg tccagtacgt gtcatggccc
      661 ccggcaacgc ccaccaggac ccccatcacc acccacagac cggggcccat ggtcgtccgt
      721 cccggctgcc agtccgcaga tgggggggtg tccgtaccca cggcccaaag aggctccgca
      781 cctcggaggc tatcggaggc cctttgttgc cgtaagcgcg ggccaaagga tggggtgggg
      841 tgagggtaaa agcacaaagg gagtaccaga ccgaaaacaa ggacggatcg gcccgctccg
      901 tttttcggtg gggtgctgat acggtgccag ccctggcccc gaacccccgc gcttatggac
      961 acaccacacg acaacaatgc cttttattct gttcttttat tgccgtcatc gccgggaggc
     1021 cttccgttcg ggcttccgtg tttgaactaa actcccccca cctcgcgggc aaacgtgcgc
     1081 gccaggtc
//
