LOCUS       sequence-73819-e        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Wu S, Ying G, Wu Q, Capecchi MR
  TITLE     A protocol for constructing gene targeting vectors: generating 
            knockout mice for the cadherin family and beyond.
  JOURNAL   Nat Protoc. 2008  . 3(6):1056-76.
  PUBMED    18546598
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2008  . 3(6):1056-76."
COMMENT     Sequence Label: pWS-TK3
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(133..257)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        502..564
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           586..625
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
ORIGIN
        1 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
       61 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      121 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
      181 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
      241 ttcttgtaca aagtggttga ttcgaggctg ctaacaaatc gagcagtgtg gttttcaaga
      301 ggaagcaaaa agcctctcca cccaggcctg gaatgtttcc acccaatgtc gagcagtgtg
      361 gttttgcaag aggaagcaaa aagcctctcc acccaggcct ggaatgtttc cacccaatgt
      421 cgagcaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg
      481 gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga
      541 gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatct tggtggcgtg
      601 aaactcccgc acctcttcgg ccagcgcctt gtagaagcgc gtatggcttc gtaccccggc
      661 catcaacacg cgtctgcgtt cgaccaggct gcgcgttctc gcggccatag caaccgacgt
      721 acggcgttgc gccctcgccg gcagcaagaa gccacggaag tccgcccgga gcagaaaatg
      781 cccacgctac tgcgggttta tatagacggt ccccacggga tggggaaaac caccaccacg
      841 caactgctgg tggccctggg ttcgcgcgac gatatcgtct acgtacccga gccgatgact
      901 tactggcggg tgctgggggc ttccgagaca atcgcgaaca tctacaccac acaacaccgc
      961 ctcgaccagg gtgagatatc ggccggggac gcggcggtgg taatgacaag cgcccagata
     1021 acaatgggca tgccttatgc cgtgaccgac gccgttctgg ctcctcatat cgggggg
//
