LOCUS       sequence-73821-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 mutant lacking cysteine residues within 
            transmembrane domains and with an N-terminal 3xFLAG-YFP tag in 
            mammalian cells. cDNA is synthetic,containing convenient restriction
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 TM-cysteineless (NT859)
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccatgtgtac gtgtccacag atcatcaaac caacattttt tgtgaaatcg tgaacaagat
       61 gaagtaaggc cggccgtgag tttggcgctc ctgtcataac aagacactgt ggcctaaagt
      121 ttttcacatg gtcctccacg ccagaaagcc taatactatg ttgcagggca ttgaggtaag
      181 tcagggcttg tgtagaggat ccccaattca catcgggttt tttgtaggta acataaatat
      241 agagaccaag gactatcacg taggttagca aagcagccca ccagttaatg acgaacatta
      301 ctatcacgga aagaattgct ccaagaagtg agatccacat attgtagtat ttaaatgccg
      361 gtcgccatcc tgggcttttt gccagtgaag catggaatac tgaaaaattg atgagggcat
      421 agctggcaag gaagaagttt gagataattg gtgcaataac attcagttcg gcaattaaga
      481 tgaatccaag tgcaattaag aatgttaaga tgtagccacg taatggctca ttatttttcc
      541 cataaccttt agcaaacatc tggaaagcgg ggtagatgtt gtctttggat agggcctgaa
      601 atatcttggg agcactcact agggatgcta atgcggaaga cagagtggca gagaatatgc
      661 ccgcggaaat tagtggtgta aatcctgaca ccatactcat tacctgaaag ttgttcatta
      721 ggccgtagct acaaggactg gattcacaag atgaaaaatc aaagtttaat ttgcaggctg
      781 cgctagtaca gtttgttagc tctgttacga tagtgtcatt aacattacca gtggcatcgc
      841 gtacgaccat agaacctaca gacacggcaa ttcctacata aaccaatgta gtaattaaaa
      901 tggctaggag tgttcctttg ggtatggctg actg
//
