LOCUS       sequence-73825-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate Compatible TALE Transcription Factor using VP64 Domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  TITLE     Bao Lab Golden Gate plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALE64
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(75..95)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(75..93)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(114..130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(114..130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(127..149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    138..154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(162..168,169..186,187..192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    207..228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             759..779
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             804..953
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcc
       61 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
      121 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      181 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      241 tcactgcccg ctttccaccg gtcgtctcca acgaccatct ggtggcgttg gcatgtcttg
      301 gtggacgacc cgcgctcgat gcagtcaaaa agggtctgcc tcatgctccc gcattgatca
      361 aaagaaccaa ccggcggatt cccgagagaa cttcccatcg agtcgcggat cacgcgcaag
      421 tggtccgcgt gctcggattc ttccagtgtc actcccaccc cgcacaagcg ttcgatgacg
      481 ccatgactca atttggtatg tcgagacacg gactgctgca gctctttcgt agagtcggtg
      541 tcacagaact cgaggcccgc tcgggcacac tgcctcccgc ctcccagcgg tgggacagga
      601 ttctccaagc gagcggtatg aaacgcgcga agccttcacc tacgtcaact cagacacctg
      661 accaggcgag ccttcatgcg ttcgcagact cgctggagag ggatttggac gcgccctcgc
      721 ccatgcatga aggggaccaa actcgcgcgt cagctagccc caagaagaag agaaaggtgg
      781 aggccagcgg ttccggacgg gctgacgcat tggacgattt tgatctggat atgctgggaa
      841 gtgacgccct cgatgatttt gaccttgaca tgcttggttc ggatgccctt gatgactttg
      901 acctcgacat gctcggcagt gacgcccttg atgatttcga cctggacatg ctgattaact
      961 ctagttaagt ttaaaccgct gatcagcc
//
