LOCUS       sequence-73826-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate Compatible TALE Transcription Factor using VP64 Domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  TITLE     Bao Lab Golden Gate plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALE64
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..166)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(191..211)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    742..763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(778..783,784..801,802..808)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    816..832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     821..843
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     840..856
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     840..856
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     875..895
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        877..895
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(942..961)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(943..961)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(971..988)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(971..987)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cttaactaga gttaatcagc atgtccaggt cgaaatcatc aagggcgtca ctgccgagca
       61 tgtcgaggtc aaagtcatca agggcatccg aaccaagcat gtcaaggtca aaatcatcga
      121 gggcgtcact tcccagcata tccagatcaa aatcgtccaa tgcgtcagcc cgtccggaac
      181 cgctggcctc cacctttctc ttcttcttgg ggctagctga cgcgcgagtt tggtcccctt
      241 catgcatggg cgagggcgcg tccaaatccc tctccagcga gtctgcgaac gcatgaaggc
      301 tcgcctggtc aggtgtctga gttgacgtag gtgaaggctt cgcgcgtttc ataccgctcg
      361 cttggagaat cctgtcccac cgctgggagg cgggaggcag tgtgcccgag cgggcctcga
      421 gttctgtgac accgactcta cgaaagagct gcagcagtcc gtgtctcgac ataccaaatt
      481 gagtcatggc gtcatcgaac gcttgtgcgg ggtgggagtg acactggaag aatccgagca
      541 cgcggaccac ttgcgcgtga tccgcgactc gatgggaagt tctctcggga atccgccggt
      601 tggttctttt gatcaatgcg ggagcatgag gcagaccctt tttgactgca tcgagcgcgg
      661 gtcgtccacc aagacatgcc aacgccacca gatggtcgtt ggagacgacc ggtggaaagc
      721 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      781 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      841 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
      901 aaagctggag gacctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt
      961 acgcgcgctc actggccgtc gttttacaac gtcgtgactg
//
