LOCUS       sequence-73830-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Rab5 fused to mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Friedman JR, Webster BM, Mastronarde DN, Verhey KJ, Voeltz GK
  TITLE     ER sliding dynamics and ER-mitochondrial contacts occur on 
            acetylated microtubules.
  JOURNAL   J Cell Biol. 2010 Aug 9;190(3):363-75. doi: 10.1083/jcb.200911024.
  PUBMED    20696706
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2010-08-9- 9"; volume: "190"; 
            issue: "3"; pages: "363-75"
COMMENT     Sequence Label: mCh-Rab5
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..752
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(190..208)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     458..477
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcatggtg agcaagggcg
       61 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
      121 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      181 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
      241 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
      301 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
      361 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
      421 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
      481 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
      541 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
      601 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
      661 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
      721 actccaccgg cggcatggac gagctgtaca agagatccac tagcagaagc acagctaggc
      781 ccaatgggca accccaggcc agcaaaattt gccagttcaa attggtcctg ctgggagaat
      841 ctgcagtggg aaagtcaagc ctggtattac gttttgtcaa agggcagttc catgagtacc
      901 aggagagcac cattggagcg gccttcctca cccagtccgt ttgtctagat gacacaacag
      961 tgaagtttga gatctgggac acagctgggc aggagcg
//
