LOCUS       sequence-73839-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPXL
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    116..233
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     intron          290..493
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(313..333)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtagcg ctaggacgcg
       61 taagcttagg cctggatccc gtagtttaaa cctcgaggct agtctcgtga tcgataaaat
      121 tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt
      181 ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta aaagttaacc
      241 gataccgtcg agattaatta aatttaaatg tcgaaattcc tcacgacacc tgaaatggaa
      301 gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa
      361 gggcaaattc caaggagaat tacatcaagt gccaagctgg cctaacttca gtctccaccc
      421 actcagtgtg gggaaactcc atcgcataaa acccctcccc ccaacctaaa gacgacgtac
      481 tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac
      541 atgaagcgac ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc
      601 gctctcccga gcgccgcgtc ctccattttg agctccctgc agcagggccg ggaagcggcc
      661 atctttccgc tcacgcaact ggtgccgacc gggccagcct tgccgcccag ggcggggcga
      721 tacacggcgg cgcgaggcca ggcaccagag caggccggcc agcttgagac tacccccgtc
      781 cgattctcgg tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac
      841 gggccccgtc gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga tcttggcccg
      901 catttacaag actatcttgc cagaaaaaaa gcgtcgcagc aggtcatcaa aaattttaaa
      961 tggctagaga cttatcgaaa gcagcgagac aggcgc
//
