LOCUS       sequence-73844-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pMT mir-1-1 reporter cel-mir-40
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(332..356)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             370..411
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             770..787
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(810..827)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(853..872)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     907..926
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aaaaagcagg ctcatatgcc agctgatttc gtcttggtaa taagctcgtc attgagatta
       61 tcaccgggtg taaatcagct tggctctggt gtctccgaac ctcctgtccg cacctcagtg
      121 gatgtatgcc atgatgataa gatatcagaa atcctatcac cgggtgtaca tcagctaagg
      181 tgcgggtaca ggtgcatttg atatcaaggt gagttatttt taagtgggtc ccagagacct
      241 tggtggtttt tctctgcagt gatagatact tctaacaact cgctatcacc gggtgaaaaa
      301 tcacctaggt ctggagcctc ctgctaagct tacccagctt tcttgtacaa agtggttcga
      361 tggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgttctaga
      421 gcagtggggt cagcttctac cggggcggcg tcccggggtc ttggaactgc atgcagactg
      481 cctgcttggg aaacatactt ctttatatgc ccatatggac ctgctaagct atggaatgta
      541 aagaagtatg tatctcaggc cgggacctct ctcgccgcac tgaggggcac tccacaccac
      601 gggggccgcc gcggccgaca gccagccagg cggctgtggt gggggtgagg ctggggcggc
      661 aggctgcctg ggcgttggtg gacggtcgtg tccccgacag cattgttctg tcactttgcc
      721 tgtgtcagca ggtggaagtg ctcaccagac atctagaacg cgtaccggtc atcatcacca
      781 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctaaga tccagacatg
      841 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      901 atttgtgaaa tttgtgatgc tattgcttta tttgtaacc
//
