LOCUS       sequence-73845-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir44-wt
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    50..74
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(252..276)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             629..670
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(710..729)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    754..802
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttaatacgac tcactatagg gagacccaag ctggctagtt aagctatcaa caagtttgta
       61 caaaaaagca ggctgtcgcg ggcggtgctg agtttaaggc ctctgaaaag agaaaatggc
      121 caatctggat gtgctcgttg gtcatagacg tcaacacgaa ctgttcatat gactagagac
      181 acattcagct tggcctgctt ctctagtttt gaatttttca acagtaagtg attaactaac
      241 aaaacttaga tgcccagctt tcttgtacaa agtggttgat ctagagggcc cgcagtgggg
      301 tcagcttcta ccggggcggc gtcccggggt cttggaactg catgcagact gcctgcttgg
      361 gaaacatact tctttatatg cccatatgga cctgctaagc tatggaatgt aaagaagtat
      421 gtatctcagg ccgggacctc tctcgccgca ctgaggggca ctccacacca cgggggccgc
      481 cgcggccgac agccagccag gcggctgtgg tgggggtgag gctggggcgg caggctgcct
      541 gggcgttggt ggacggtcgt gtccccgcca gcattgttct gtcactttgc ctgtgtcagc
      601 aggtggaagt gctcaccaga cattcgaagg taagcctatc cctaaccctc tcctcggtct
      661 cgattctacg cgtaccggtt agtaatgagt ttaaacgggg gaggctaact gaaacacgga
      721 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
      781 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
      841 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
      901 caccccccaa gttcgggtg
//
