LOCUS       sequence-73850-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Goldstein JC, Waterhouse NJ, Juin P, Evan GI, Green DR
  TITLE     The coordinate release of cytochrome c during apoptosis is rapid, 
            complete and kinetically invariant.
  JOURNAL   Nat Cell Biol. 2000 Mar;2(3):156-62.
  PUBMED    10707086
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2000-03"; volume: "2(3)"; pages: "156-62"
COMMENT     Sequence Label: pBabe(SV40)-Cytochrome C-GFP
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      355..364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(371..700)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      385..520
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     685..705
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(779..795)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(811..833)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc tttctcatta gtagcctttt
       61 taagataagc tattaggtct gccctttctc ccttcttctt aattccagcg aagatcattt
      121 ttgttccagg gatgtacttt ttgggattct ccaaatactc catcagggta tcctctcccc
      181 aggtgatgcc tttgttcttg ttggcatctg tgtaagagaa tccagcagcc tggcctgtct
      241 tccgcccgaa cagaccgtgg agatttggtc cagtcttatg cttgcctccc ttttccacag
      301 tgtggcactg ggcacacttc tgaacaaaaa tcttcttgcc tttttcaaca tcacccatgg
      361 tggcaagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
      421 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
      481 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
      541 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
      601 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
      661 ctggggagcc tggggacttt ccacacccta actgacacac attccacagg gtcgaccact
      721 gtgctggcga attcctacgt accaccacac tgggatccgg ccggcgccta gagaaggagt
      781 gagggctgga taaagggagg atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga
      841 cggggcggat ggaggaagag gaggcggagg cttagggtgt acaaagggct tgacccaggg
      901 aggggggtca aaagccaagg cttcccaggt cacgatgtag ggacctggtc tgggtgtcca
      961 tgc
//
