LOCUS       sequence-73852-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of GFP nano body.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Kubala MH, Kovtun O, Alexandrov K, Collins BM
  TITLE     Structural and thermodynamic analysis of the GFP:GFP-nanobody 
            complex.
  JOURNAL   Protein Sci. 2010 Dec;19(12):2389-401. doi: 10.1002/pro.519.
  PUBMED    20945358
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro"; 
            journalName: "Protein Sci"; date: "2010-12"; volume: "19"; issue: 
            "12"; pages: "2389-401"
COMMENT     Sequence Label: pOPINE GFP nanobody
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        14..123
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             144..485
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     CDS             489..506
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(596..615)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    661..716
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(715..734)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      804..851
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggagttaatc cgggaccttt aattcaaccc aacacaatat attatagtta aataagaatt
       61 attatcaaat catttgtata ttaattaaaa tactatactg taaattacat tttatttaca
      121 atcaaaggag atataccatg caggttcaac tggtggaaag cggcggtgct ctggtacaac
      181 cgggcggtag tctgcgcctg agctgtgccg caagcggttt cccagtcaac cgctactcta
      241 tgcgttggta tcgccaggcg cctggtaaag aacgtgaatg ggttgccggc atgagcagtg
      301 cgggcgatcg ttctagttac gaggactctg ttaaaggtcg ttttacaatt agccgtgatg
      361 atgcgcgcaa taccgtgtat ctgcaaatga acagtctgaa gccggaggac accgcagtat
      421 attattgcaa tgtcaacgtg gggtttgaat attggggcca ggggactcag gtgacggtga
      481 gctctaaaca tcaccatcac catcactaag tgattaacct caggtgcagg ctgcctatca
      541 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atcgatcttt
      601 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
      661 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
      721 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
      781 tggcaacata tgcccatatg taactagcat aaccccttgg ggcctctaaa cgggtcttga
      841 ggggtttttt gctgaaagca tgcgga
//
