LOCUS       sequence-73853-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing AP-Sstr3-GFP in mammalian cells, can be used for 
            establishing Flp-In stable cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Ye F, Breslow DK, Koslover EF, Spakowitz AJ, Nelson WJ, Nachury MV
  TITLE     Single molecule imaging reveals a major role for diffusion in the 
            exploration of ciliary space by signaling receptors.
  JOURNAL   Elife. 2013 Aug 6;2:e00654. doi: 10.7554/eLife.00654. Print 2013.
  PUBMED    23930224
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2013-08-6"; volume: "2:e00654. doi"; pages: " 
            10.7554/eLife.00654. Print 2013"
COMMENT     Sequence Label: pEF5B-FRT-AP-Sstr3-GFP-DEST
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             85..129
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 agcttggtac cgagctcgga tccactagtc cagtgtggtg gaattctgca gatatcaaca
       61 agtttgtaca aaaaagttga tatgggcctg aacgacatct tcgaggctca gaaaatcgaa
      121 tggcacgaag ccactgttac ctatccctca tccgagccta tgaccttgga ccctgggaac
      181 acatcctcga cctggcccct ggataccacc ctggggaaca catccgctgg cgctagcctg
      241 acaggcctgg ctgtcagtgg catcttgatc tctctggtgt acctggtggt gtgcgtggtg
      301 ggtctgctgg gcaactcgct ggtgatctac gtggtcctgc ggcacacgtc cagcccatca
      361 gtgaccagtg tctatatcct caacctggct ctggctgatg agctcttcat gctagggcta
      421 cccttcctgg ctgctcagaa cgccctgtcc tactggccct ttggatctct catgtgccgt
      481 ctggtcatgg ccgtggatgg catcaaccag ttcaccagca tcttctgcct caccgtcatg
      541 agtgtggacc gctatctggc tgtggtgcac cccacacgct cagcccgctg gcgcacggca
      601 ccagtggctc gcacggtcag tgcagctgtc tgggtggcct cggctgtggt ggtgctgcct
      661 gtggttgtgt tctcaggagt gccccggggc atgagcacgt gccacatgca gtggccagag
      721 ccagcggctg cctggcgaac agcctttatc atctacacgg ccgcactggg cttctttggg
      781 cccctgctgg tcatctgctt gtgctacttg ctcattgtgg taaaggtgcg gtcgaccacc
      841 cggcgggtgc gggcgccctc gtgtcagtgg gtacaggcac ccgcatgcca gcggcgacgc
      901 cgctctga
//
