LOCUS       sequence-73857-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing AP-Smo-YFP in mammalian cells, can be used for 
            establishing Flp-In stable cell lines .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Ye F, Breslow DK, Koslover EF, Spakowitz AJ, Nelson WJ, Nachury MV
  TITLE     Single molecule imaging reveals a major role for diffusion in the 
            exploration of ciliary space by signaling receptors.
  JOURNAL   Elife. 2013 Aug 6;2:e00654. doi: 10.7554/eLife.00654. Print 2013.
  PUBMED    23930224
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2013-08-6"; volume: "2:e00654. doi"; pages: " 
            10.7554/eLife.00654. Print 2013"
COMMENT     Sequence Label: pEF5B-FRT-AP-Smo-YFP-DEST
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             256..300
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 agcttggtac cgagctcgga tccactagtc cagtgtggtg gaattctgca gatatcaaca
       61 agtttgtaca aaaaagttga tatggccgct ggccgccccg tgcgtgggcc cgagctggcg
      121 ccccggaggc tgctgcagtt gctgctgctg gtactgctgg ggggcccggg ccggggggcg
      181 gccttgagcg ggaacgtgac cgggcctggg cctcacagcg ccagcgggag ctcgaggagg
      241 aacgtgccgg tgaccggcct gaacgatatc ttcgaggccc agaagatcga gtggcacgag
      301 gtgaccagcc ctccgccgcc gctgctgagc cactgcggcc gggccgccca ctgcgagcct
      361 ttgcgctaca acgtgtgcct gggctcggcg ctgccctacg gagccaccac cacgctgctg
      421 gctggggact cggactcgca ggaggaagcg cacggcaagc tcgtgctctg gtccggcctc
      481 cggaatgccc cccgctgctg ggcagtgatc cagcccctgc tgtgtgctgt ctacatgccc
      541 aagtgtgaga atgaccgagt ggagttgccc agccgtaccc tctgccaggc cacccgaggc
      601 ccctgtgcca ttgtggagcg ggagcgaggg tggcctgact ttctgcgttg cacaccggac
      661 cacttccctg aaggctgccc aaacgaggta caaaacatca agttcaacag ctcaggccaa
      721 tgtgaagcac ccttggtgcg aacagacaac cccaagagct ggtatgagga cgtggagggc
      781 tgtgggattc agtgtcagaa cccgctgttc accgaggccg agcaccagga catgcacagc
      841 tacatcgcag ccttcggggc ggtcactggt ctctgcacgc tcttcacact ggccaccttt
      901 gtggctgact gg
//
