LOCUS       sequence-73860-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter hsa-mir17~20
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagtt aagctatcaa caagtttgta caaaaaagca ggctcatatg ccccattagg
      121 gattatgctg aatttgtatg gtttatagtt gttagagttt gaggtgttaa ttctaattat
      181 ctatttcaaa tttagcagga aaaaagagaa catcaccttg taaaactgaa gattgtgacc
      241 agtcagaata atgtcaaagt gcttacagtg caggtagtga tatgtgcatc tactgcagtg
      301 aaggcacttg tagcattatg gtgacagctg cctcgggaag ccaagttggg ctttaaagtg
      361 cagggcctgc tgatgttgag tgctttttgt tctaaggtgc atctagtgca gatagtgaag
      421 tagattagca tctactgccc taagtgctcc ttctggcata agaagttatg tattcatcca
      481 ataattcaag ccaagcaagt atataggtgt tttaatagtt tttgtttgca gtcctctgtt
      541 agttttgcat agttgcacta caagaagaat gtagttgtgc aaatctatgc aaaactgatg
      601 gtggcctgct atttccttca aatgaatgat ttttactaat tttgtgtact tttattgtgt
      661 cgatgtagaa tctgcctggt ctatctgatg tgacagcttc tgtagcacta aagtgcttat
      721 agtgcaggta gtgtttagtt atctactgca ttatgagcac ttaaagtact gctagctgta
      781 gaactccagc ttcggcctgt cgcccaatca aactgtcctg ttactgaaca ctgttctatg
      841 gttagttttg caggtttgca tccagctgtg tgatattctg ctgtgcaaat ccatgcaaaa
      901 ctgactgtgg tagtgaaaag tctgtagaaa agta
//
