LOCUS       sequence-73863-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir50-1
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8..26
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(262..286)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             639..680
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(720..739)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    764..812
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagttaagc tatcaacaag
       61 tttgtacaaa aaagcaggct ctatttttac taattctgaa ttccataatt ctatattctg
      121 tattccaccc cgccggccgc tgatatgtct ggtattcttg ggtttgaact tccagcgttg
      181 aacccgcata ttagacgtat cgacggccgg cggggcataa tggctggtaa gtttggagag
      241 accgggggag tcccagagat gccccagctt tcttgtacaa agtggttgat ctagagggcc
      301 cgcagtgggg tcagcttcta ccggggcggc gtcccggggt cttggaactg catgcagact
      361 gcctgcttgg gaaacatact tctttatatg cccatatgga cctgctaagc tatggaatgt
      421 aaagaagtat gtatctcagg ccgggacctc tctcgccgca ctgaggggca ctccacacca
      481 cgggggccgc cgcggccgac agccagccag gcggctgtgg tgggggtgag gctggggcgg
      541 caggctgcct gggcgttggt ggacggtcgt gtccccgcca gcattgttct gtcactttgc
      601 ctgtgtcagc aggtggaagt gctcaccaga cattcgaagg taagcctatc cctaaccctc
      661 tcctcggtct cgattctacg cgtaccggtt agtaatgagt ttaaacgggg gaggctaact
      721 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
      781 aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt ccc
//
