LOCUS       sequence-73864-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir30-3
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     ncRNA           167..181
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(259..283)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             636..677
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(717..736)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    761..809
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagttaagct
       61 atcaacaagt ttgtacaaaa aagcaggctg gttaacccaa cagaaggcta aagaaggtat
      121 attgctgttg acagtgagcg actgtaaaca tcctcgactg gaagctgtga agccacagat
      181 gggctttcag tcggatgttt gcagctgcct actgcctcgg actttaaggg gctactttag
      241 gagcaattat cttgtttacc ccagctttct tgtacaaagt ggttgatcta gagggcccgc
      301 agtggggtca gcttctaccg gggcggcgtc ccggggtctt ggaactgcat gcagactgcc
      361 tgcttgggaa acatacttct ttatatgccc atatggacct gctaagctat ggaatgtaaa
      421 gaagtatgta tctcaggccg ggacctctct cgccgcactg aggggcactc cacaccacgg
      481 gggccgccgc ggccgacagc cagccaggcg gctgtggtgg gggtgaggct ggggcggcag
      541 gctgcctggg cgttggtgga cggtcgtgtc cccgccagca ttgttctgtc actttgcctg
      601 tgtcagcagg tggaagtgct caccagacat tcgaaggtaa gcctatccct aaccctctcc
      661 tcggtctcga ttctacgcgt accggttagt aatgagttta aacgggggag gctaactgaa
      721 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
      781 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
      841 ctctgtcgat accccaccga gaccccattg ggg
//
