LOCUS       sequence-73865-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pMT mir-1-1 reporter cel-lin-4
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(375..399)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             413..454
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             813..830
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(853..870)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(896..915)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aaaaagcagg ctcatatgct accacaatcg gtcggactca tcacacttac ctttcaaata
       61 tctaaactat tcctgaatat aataaatctt atagttttta gattctagac aatttctaga
      121 gttttggttg gtttatgagt ttatgcttcc ggcctgttcc ctgagacctc aagtgtgagt
      181 gtactattga tgcttcacac ctgggctctc cgggtaccag gacggtttga gcagatcttt
      241 ttttctgttt tcacggggtt ttttttcaca ctgggagcta gttccaataa aatggtattc
      301 ggggaaaagg gtcaaacgat tattttcaga agtcgactac aatagagttc cactaacgcc
      361 aactgatgaa gcttacccag ctttcttgta caaagtggtt cgatggttcg aaggtaagcc
      421 tatccctaac cctctcctcg gtctcgattc tacgcgttct agagcagtgg ggtcagcttc
      481 taccggggcg gcgtcccggg gtcttggaac tgcatgcaga ctgcctgctt gggaaacata
      541 cttctttata tgcccatatg gacctgctaa gctatggaat gtaaagaagt atgtatctca
      601 ggccgggacc tctctcgccg cactgagggg cactccacac cacgggggcc gccgcggccg
      661 acagccagcc aggcggctgt ggtgggggtg aggctggggc ggcaggctgc ctgggcgttg
      721 gtggacggtc gtgtccccga cagcattgtt ctgtcacttt gcctgtgtca gcaggtggaa
      781 gtgctcacca gacatctaga acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa
      841 cccgctgatc agcctcgact gtgccttcta agatccagac atgataagat acattgatga
      901 gtttggacaa accacaacta
//
