LOCUS       sequence-73867-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir30-7
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     ncRNA           164..178
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(256..280)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             633..674
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(714..733)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    758..806
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ggcttatcga aattaatacg actcactata gggagaccca agctggctag ttaagctatc
       61 aacaagtttg tacaaaaaag caggctggtt aacccaacag aaggctaaag aaggtatatt
      121 gctgttccgc gtgagcgact gtaaacatcc tcgactggaa gctgtgaagc cacagatggg
      181 ctttcagtcg gatgtttgca gctgcctact gcctcggact tcaaggggct actttaggag
      241 caattatctt gtttacccca gctttcttgt acaaagtggt tgatctagag ggcccgcagt
      301 ggggtcagct tctaccgggg cggcgtcccg gggtcttgga actgcatgca gactgcctgc
      361 ttgggaaaca tacttcttta tatgcccata tggacctgct aagctatgga atgtaaagaa
      421 gtatgtatct caggccggga cctctctcgc cgcactgagg ggcactccac accacggggg
      481 ccgccgcggc cgacagccag ccaggcggct gtggtggggg tgaggctggg gcggcaggct
      541 gcctgggcgt tggtggacgg tcgtgtcccc gccagcattg ttctgtcact ttgcctgtgt
      601 cagcaggtgg aagtgctcac cagacattcg aaggtaagcc tatccctaac cctctcctcg
      661 gtctcgattc tacgcgtacc ggttagtaat gagtttaaac gggggaggct aactgaaaca
      721 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
      781 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
      841 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
      901 accccacccc ccaagttcgg gtgaaggccc agg
//
