LOCUS       sequence-73868-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir44-5
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(256..280)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             633..674
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(714..733)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    758..806
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gaaattaata cgactcacta tagggagacc caagctggct agttaagcta tcaacaagtt
       61 tgtacaaaaa agcaggctgt cgcgggcggt gctgagttta aggcctctga aaagagaaaa
      121 tggccaatct ggatgtgctc gttggtcata gacgtcaaca cgaactgttc atatgactag
      181 agacacattc agcttggcct gcttctctag cttcgaattt ttcaacagta agtgattaac
      241 taacaaaact tagatgccca gctttcttgt acaaagtggt tgatctagag ggcccgcagt
      301 ggggtcagct tctaccgggg cggcgtcccg gggtcttgga actgcatgca gactgcctgc
      361 ttgggaaaca tacttcttta tatgcccata tggacctgct aagctatgga atgtaaagaa
      421 gtatgtatct caggccggga cctctctcgc cgcactgagg ggcactccac accacggggg
      481 ccgccgcggc cgacagccag ccaggcggct gtggtggggg tgaggctggg gcggcaggct
      541 gcctgggcgt tggtggacgg tcgtgtcccc gccagcattg ttctgtcact ttgcctgtgt
      601 cagcaggtgg aagtgctcac cagacattcg aaggtaagcc tatccctaac cctctcctcg
      661 gtctcgattc tacgcgtacc ggttagtaat gagtttaaac gggggaggct aactgaaaca
      721 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
      781 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
      841 tgtcgatacc ccaccgagac cccattgggg
//
