LOCUS       sequence-73873-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir30-5
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    91..115
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     ncRNA           193..207
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(285..309)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             662..703
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(743..762)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    787..835
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
       61 ggagacccaa gctggctagt taagctatca acaagtttgt acaaaaaagc aggctggtta
      121 acccaacaga aggctaaaga aggtatattg ctgttgacag tgagcgactg taaacatcct
      181 cgactggaag ctgtgaagcc acagatgggc tttcagtcgg atgtttgcag ctgcctactg
      241 cctcggattt taaggggcta ctttaggagc aattatcttg tttaccccag ctttcttgta
      301 caaagtggtt gatctagagg gcccgcagtg gggtcagctt ctaccggggc ggcgtcccgg
      361 ggtcttggaa ctgcatgcag actgcctgct tgggaaacat acttctttat atgcccatat
      421 ggacctgcta agctatggaa tgtaaagaag tatgtatctc aggccgggac ctctctcgcc
      481 gcactgaggg gcactccaca ccacgggggc cgccgcggcc gacagccagc caggcggctg
      541 tggtgggggt gaggctgggg cggcaggctg cctgggcgtt ggtggacggt cgtgtccccg
      601 ccagcattgt tctgtcactt tgcctgtgtc agcaggtgga agtgctcacc agacattcga
      661 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg
      721 agtttaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
      781 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
      841 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
      901 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaa
//
