LOCUS       sequence-73875-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir40-7
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(248..272)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             625..666
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(706..725)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    750..798
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ggcttatcga aattaatacg actcactata gggagaccca agctggctag ttaagctatc
       61 aacaagtttg tacaaaaaag caggctggtg taaatcagct tggctctggt gtctccgaac
      121 cttgtgtccg cacctcagtg gatgtatgcc atgatgataa gatatcagaa atcctatcac
      181 cgggtgtaca tcagctaagg tgcgggtaca catgcatctg ctatcaaggt gagttatttt
      241 taagtgggcc cagctttctt gtacaaagtg gttgatctag agggcccgca gtggggtcag
      301 cttctaccgg ggcggcgtcc cggggtcttg gaactgcatg cagactgcct gcttgggaaa
      361 catacttctt tatatgccca tatggacctg ctaagctatg gaatgtaaag aagtatgtat
      421 ctcaggccgg gacctctctc gccgcactga ggggcactcc acaccacggg ggccgccgcg
      481 gccgacagcc agccaggcgg ctgtggtggg ggtgaggctg gggcggcagg ctgcctgggc
      541 gttggtggac ggtcgtgtcc ccgccagcat tgttctgtca ctttgcctgt gtcagcaggt
      601 ggaagtgctc accagacatt cgaaggtaag cctatcccta accctctcct cggtctcgat
      661 tctacgcgta ccggttagta atgagtttaa acgggggagg ctaactgaaa cacggaagga
      721 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
      781 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
      841 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
      901 ccccaa
//
