LOCUS       sequence-73877-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter hsa-mir-138-2
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(542..566)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagttaa gctatcaaca agtttgtaca aaaaagcagg
      121 ctcatatgct gtctcaaaac cggaggagcc acagcccaga ggaggaagcc ggcggagttc
      181 tggtatcgtt gctgcagctg gtgttgtgaa tcaggccgac gagcagcgca tcctcttacc
      241 cggctatttc acgacaccag ggttgcatca tacccatcct ctccaggcga gcctcgtggg
      301 accgggccgg acgcaaggac ctggggatgg ggttggggtg ggagtctgtg tgtgaaagct
      361 tgtcctctgt agctcactgg atctcagccc agactgctcc tcagaatcac ctggcagctt
      421 tcaaaaaact ctgatgtctg cccctcacct cctcttgccg aggtccacag aatctccaag
      481 gtggggccct ggaatcagca ttgttagagg ttccagtgtg atgccgaagt ctgtgaagct
      541 tacccagctt tcttgtacaa agtggttgat ctagagggcc cgcagtgggg tcagcttcta
      601 ccggggcggc gtcccggggt cttggaactg catgcagact gcctgcttgg gaaacatact
      661 tctttatatg cccatatgga cctgctaagc tatggaatgt aaagaagtat gtatctcagg
      721 ccgggacctc tctcgccgca ctgaggggca ctccacacca cgggggccgc cgcggccgac
      781 agccagccag gcggctgtgg t
//
