LOCUS       sequence-73879-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pMT mir-1-1 reporter hsa-mir-128-1
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(371..395)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             409..450
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             809..826
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(849..866)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 aaaaagcagg ctcatatgca aagccctagc tgttttctgt gtagctttta ttattcttat
       61 gactcttgac aagtttgtag cttcaccata tacatttaat attttgcaat aattggcctt
      121 gttcctgagc tgttggattc ggggccgtag cactgtctga gaggtttaca tttctcacag
      181 tgaaccggtc tctttttcag ctgcttcctg gcttcttttt actcaggttt ccactgcttt
      241 tttgcttttt ttaatgctgt atgaaggtgt taacatttgt ttatattttt cattaattgt
      301 aataccttta aatcatgcat catactcaga aatagggatt agaatttaag tgacatcttt
      361 ggccaagctt acccagcttt cttgtacaaa gtggttcgat ggttcgaagg taagcctatc
      421 cctaaccctc tcctcggtct cgattctacg cgttctagag cagtggggtc agcttctacc
      481 ggggcggcgt cccggggtct tggaactgca tgcagactgc ctgcttggga aacatacttc
      541 tttatatgcc catatggacc tgctaagcta tggaatgtaa agaagtatgt atctcaggcc
      601 gggacctctc tcgccgcact gaggggcact ccacaccacg ggggccgccg cggccgacag
      661 ccagccaggc ggctgtggtg ggggtgaggc tggggcggca ggctgcctgg gcgttggtgg
      721 acggtcgtgt ccccgacagc attgttctgt cactttgcct gtgtcagcag gtggaagtgc
      781 tcaccagaca tctagaacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
      841 ctgatcagcc tcgactgtgc cttctaagat ccagacatga taagatacat tgatgagttt
      901 g
//
