LOCUS       sequence-73882-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir40-1
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    50..74
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(236..260)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             613..654
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(694..713)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    738..786
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttaatacgac tcactatagg gagacccaag ctggctagtt aagctatcaa caagtttgta
       61 caaaaaagca ggctggtgta aatcagcttg gctctggtgt ctccgaacct tgtgtccgca
      121 cctcagtgga tgtatgccat gatgataaga tatcagaaat cctatcaccg ggtgtacatc
      181 agctaaggtg cgggtacagg tgcatttgat atcaaggtga gttattttta agtgggccca
      241 gctttcttgt acaaagtggt tgatctagag ggcccgcagt ggggtcagct tctaccgggg
      301 cggcgtcccg gggtcttgga actgcatgca gactgcctgc ttgggaaaca tacttcttta
      361 tatgcccata tggacctgct aagctatgga atgtaaagaa gtatgtatct caggccggga
      421 cctctctcgc cgcactgagg ggcactccac accacggggg ccgccgcggc cgacagccag
      481 ccaggcggct gtggtggggg tgaggctggg gcggcaggct gcctgggcgt tggtggacgg
      541 tcgtgtcccc gccagcattg ttctgtcact ttgcctgtgt cagcaggtgg aagtgctcac
      601 cagacattcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
      661 ggttagtaat gagtttaaac gggggaggct aactgaaaca cggaaggaga caataccgga
      721 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
      781 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
      841 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
      901 gtgaa
//
