LOCUS       sequence-73888-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir40-3
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(270..294)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             647..688
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(728..747)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    772..820
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctgg tgtaaatcag
      121 cttggctctg gtgtctccga accttgtgtc cgcacctcag tggatgtatg ccatgatgat
      181 aagatatcag aaatcctatc accgggtgta catcagctaa ggtgcgggta cacatccact
      241 tgatatcaag gtgagttatt tttaagtggg cccagctttc ttgtacaaag tggttgatct
      301 agagggcccg cagtggggtc agcttctacc ggggcggcgt cccggggtct tggaactgca
      361 tgcagactgc ctgcttggga aacatacttc tttatatgcc catatggacc tgctaagcta
      421 tggaatgtaa agaagtatgt atctcaggcc gggacctctc tcgccgcact gaggggcact
      481 ccacaccacg ggggccgccg cggccgacag ccagccaggc ggctgtggtg ggggtgaggc
      541 tggggcggca ggctgcctgg gcgttggtgg acggtcgtgt ccccgccagc attgttctgt
      601 cactttgcct gtgtcagcag gtggaagtgc tcaccagaca ttcgaaggta agcctatccc
      661 taaccctctc ctcggtctcg attctacgcg taccggttag taatgagttt aaacggggga
      721 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
      781 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      841 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      901 tcttcctttt ccccacccca ccccccaagt tcgggtga
//
