LOCUS       sequence-73891-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir50-4
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(259..283)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             636..677
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(717..736)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    761..809
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gaaattaata cgactcacta tagggagacc caagctggct agttaagcta tcaacaagtt
       61 tgtacaaaaa agcaggctct atttttacta attctgaatt ccataattct atattctgta
      121 ttcctccccg ccggccgctg atatgtctgg tattcttggg tttgaacttc cagcgttgaa
      181 cccgcatatt agacgtatcg acggccggcg gggcataatg ctgctaagtt tggagagacc
      241 gggggagtcc cagagatgcc ccagctttct tgtacaaagt ggttgatcta gagggcccgc
      301 agtggggtca gcttctaccg gggcggcgtc ccggggtctt ggaactgcat gcagactgcc
      361 tgcttgggaa acatacttct ttatatgccc atatggacct gctaagctat ggaatgtaaa
      421 gaagtatgta tctcaggccg ggacctctct cgccgcactg aggggcactc cacaccacgg
      481 gggccgccgc ggccgacagc cagccaggcg gctgtggtgg gggtgaggct ggggcggcag
      541 gctgcctggg cgttggtgga cggtcgtgtc cccgccagca ttgttctgtc actttgcctg
      601 tgtcagcagg tggaagtgct caccagacat tcgaaggtaa gcctatccct aaccctctcc
      661 tcggtctcga ttctacgcgt accggttagt aatgagttta aacgggggag gctaactgaa
      721 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
      781 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
      841 ctctgtc
//
