LOCUS       sequence-73893-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic G9-5FLAG epitope tagging in S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-G9-5FLAG-hphMX6
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             72..203
                     /codon_start=1
                     /product="five tandem FLAG(R) epitope tags"
                     /label=5xFLAG
                     /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
     terminator      230..417
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(285..309)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        492..835
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 cttcgtacgc tgcaggtcga cggatccccg ggggaggtgg aggtggaggt ggaggtttaa
       61 ttaatcatat ggactacaag gacgacgatg acaaggatta caaagatgac gacgataagc
      121 ttatggacta caaggacgac gatgacaagg attacaaaga tgacgacgat aagcttatgg
      181 actacaagga cgacgatgac aagcatatgg gcgcgccact tctaaataag cgaatttctt
      241 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
      301 aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt
      361 caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct ctaccggcag
      421 atccgctagg gataacaggg taatatagat ctgtttagct tgcctcgtcc ccgccgggtc
      481 acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct
      541 caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat
      601 ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc
      661 ggacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc
      721 ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc
      781 cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa caaccatggg
      841 taaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
      901 cgtctccgac ctgatgcggc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
      961 aggagggcgt ggatatgtcc tgcgggtaaa t
//
