LOCUS       sequence-73897-e         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic 12Pk epitope tagging in S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-12Pk-hphMX6
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..656
                     /codon_start=1
                     /product="12 tandem copies of the Pk (V5) epitope"
                     /label=12Pk tag
                     /translation="GKPIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGL
                     DSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGK
                     PIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGL
                     DSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGLDST"
ORIGIN
        1 aagcttcgta cgctgcaggt cgacggatcc ccgggttaat taacctcgac ggtggaggta
       61 agcctatccc taaccctctg ctgggtcttg attctacatc cggaggtaag cctatcccta
      121 accctctgct gggtcttgat tctacactcg acggtggagg taagcctatc cctaaccctc
      181 tgctgggtct tgattctaca tccggaggta agcctatccc taaccctctg ctgggtcttg
      241 attctacact cgacggtgga ggtaagccta tccctaaccc tctgctgggt cttgattcta
      301 catccggagg taagcctatc cctaaccctc tgctgggtct tgattctaca ctcgacggtg
      361 gaggtaagcc tatccctaac cctctgctgg gtcttgattc tacatccgga ggtaagccta
      421 tccctaaccc tctgctgggt cttgattcta cactcgacgg tggaggtaag cctatcccta
      481 accctctgct gggtcttgat tctacatccg gaggtaagcc tatccctaac cctctgctgg
      541 gtcttgattc tacactcgac ggtggaggta agcctatccc taaccctctg ctgggtcttg
      601 attctacatc cggaggtaag cctatcccta accctctgct gggtcttgat tctacactcg
      661 a
//
