LOCUS       sequence-73903-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter hsa-mir-205
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(537..561)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagtt aagctatcaa caagtttgta caaaaaagca ggctcatatg agcctccttg
      121 gaggatgtga tcctatggga ggggtaggag tattcaggtc cttgacatct cccaaatgtg
      181 tgattccggg atgccaaagg cctttggcca ggtaatgcag tgtctacagg ctgaggttga
      241 catgcatccc caccctctga gaaaaagatc ctcagacaat ccatgtgctt ctcttgtcct
      301 tcattccacc ggagtctgtc tcatacccaa ccagatttca gtggagtgaa gttcaggagg
      361 catggagctg acaaccatga ggcctcggca gccaccgcca ccaccgccgc cgccaccacc
      421 gtagcagcag cagcagcagc agcagcagca gcagcagcag caagagtaac tctgacttag
      481 gaatagagac agccagagag aaatgtgatc aatgaaagag acatctggag aagcttaccc
      541 agctttcttg tacaaagtgg ttgatctaga gggcccgcag tggggtcagc ttctaccggg
      601 gcggcgtccc ggggtcttgg aactgcatgc agactgcctg cttgggaaac atacttcttt
      661 atatgcccat atggacctgc taagctatgg aatgtaaaga agtatgtatc tcaggccggg
      721 acctctctcg ccgcactgag gggcactcca caccacgggg gccgccgcgg ccgacagcca
      781 gccaggcggc tgtggtgggg gtgaggctgg ggcggcaggc tgcctgggcg ttggtggacg
      841 gtcgtgtccc cgccagcatt gttctgtcac tttgcctgtg tcagcaggtg gaagtgctca
      901 cca
//
