LOCUS       sequence-73922-e        1691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Thestrup T, Litzlbauer J, Bartholomaus I, Mues M, Russo L, Dana H, 
            Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, 
            Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger
            C, Griesbeck O
  TITLE     Optimized ratiometric calcium sensors for functional in vivo imaging
            of neurons and T lymphocytes.
  JOURNAL   Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773.
  PUBMED    24390440
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773."
COMMENT     Sequence Label: Twitch-2B pcDNA3
FEATURES             Location/Qualifiers
     source          1..1691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(60..81)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(321..340)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccgcggccgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
       61 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      121 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      181 tgccctggcc caccctcgtg accaccctga gctggggcgt gcagtgcttc gcccgctacc
      241 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      301 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      361 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      421 acatcctggg gcacaagctg gagtacaacg ccatccacgg caacgtctat atcaccgccg
      481 acaagcagaa gaacggcatc aaggccaact tcggcctcaa ctgcaacatc gaggacggca
      541 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      601 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
      661 gcgatcacat ggtcctgctg gagttcgtga ccgccgcccg catgcaagtt gccgacgcca
      721 gcgaagagga actgagcgag tgcttcagaa tcttcgactt cgacggcaac ggcttcatcg
      781 acagagagga gtttggcgac atcatcagac tgaccggcga gcagctgacc gacgaggacg
      841 tggacgagat cttcggcgac tccgacaccg acaagaacgg cagaatcgat ttcgacgagt
      901 tcctgaagat ggtggaaaac gtgcagccca tctaccccga gctcatgggt ggggtccagc
      961 ttgcagatca ctaccagcag aatactccga tcggggatgg gccggttctc ctgcctgaca
     1021 accactacct gtcataccag tccaagcttt ccaaagatcc taatgaaaag cgggatcaca
     1081 tggtcctgct ggaatttgtc accgccgccg gaatcactct cggaatggac gagctgtaca
     1141 aaggtgggac cgggggatct atggtgagca agggcgaaga attgttcact ggtgtagtcc
     1201 ctattttggt ggaactggat ggggatgtca acggtcataa attcagcgtg cgaggggagg
     1261 gtgaggggga cgcaactaat ggcaaattga cactcaagct catctgcaca acggggaaac
     1321 tgcccgtgcc atggcccaca ctcgttacta ctctaggata tggccttatg tgtttcgccc
     1381 gctacccaga ccatatgaaa caacacgatt tctttaaatc agccatgcca gaggggtacg
     1441 tccaggagag aactatcttc ttcaaggacg atggtaatta caagactagg gccgaggtga
     1501 aattcgaagg ggatacactt gtcaaccgaa tcgagctgaa gggaatcgac tttaaggagg
     1561 acggaaacat actcgggcat aagttggaat acaactacaa cagtcacaat gtttacatca
     1621 cagccgataa gcaaaagaac ggcattaagg ctaattttaa gatccgtcac aacattgagg
     1681 attgagaatt c
//
