LOCUS       sequence-73946-e        7930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses GAL4BD-Rab4BQ67LdeltaCGCin yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Cortes C, Rzomp KA, Tvinnereim A, Scidmore MA, Wizel B
  TITLE     Chlamydia pneumoniae inclusion membrane protein Cpn0585 interacts 
            with multiple Rab GTPases.
  JOURNAL   Infect Immun. 2007 Dec;75(12):5586-96. Epub 2007 Oct 1.
  PUBMED    17908815
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2007-12"; volume: "75(12)"; pages: "5586-96. Epub 
            2007 Oct 1"
COMMENT     Sequence Label: GBK-Rab4BQ67LdeltaGCG
FEATURES             Location/Qualifiers
     source          1..7930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      1964..2011
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2038..2225
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2093..2117)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2249..2265)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2249..2265)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2262..2284)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2273..2289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2297..2303,2304..2321,2322..2327))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(2480..2497)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2651..3239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2731..2750)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3568..3615
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3640..3659
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3848..4642)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3959..3978)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4569..4588
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4643..4747)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4774..6116
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(5737..5784)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6157..6175
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6213..6235)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6335..6354
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6375..6656
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6657..7331
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7434..7889)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7571..7592)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7783..7802
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga
      421 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg
      481 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct
      541 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcaccatg gctgagacct
     1321 acgacttcct cttcaaattc ctggtgattg gcagtgcagg aactggcaaa tcatgtctcc
     1381 ttcatcagtt cattgagaat aagttcaaac aggactccaa ccacacaatc ggcgtggagt
     1441 ttggatcccg ggtggtcaac gtgggtggga agactgtgaa gctacagatt tgggacacgg
     1501 ctggccatgg agcggtttcg gtcagtgacg cggagttatt accgaggggc ggctggagcc
     1561 ctgctggtgt acgacatcac cagccgggag acatacaact cactggctgc ctggctgacg
     1621 gatgcccgca ccctggccag ccccaacatc gtggtcatcc tctgtggcaa caagaaggac
     1681 ctggaccctg agcgggaggt cactttcctg gaggcctccc gctttgccca ggagaatgag
     1741 ctgatgttcc tggagaccag cgctctcaca ggcgagaacg tggaggaggc gttcctcaag
     1801 tgtgcccgca ctatcctcaa caagattgac tcaggcgagc tagacccgga gaggatgggc
     1861 tctggcattc agtacgggga tgcgtccctc cgccagcttc ggcagcctcg gagtgcccag
     1921 gccgtggccc ctcagccgga ctcgacctgc agcggccgca taactagcat aaccccttgg
     1981 ggcctctaaa cgggtcttga ggggtttttt gcgcgcttgc agccaagcta attccgggcg
     2041 aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac
     2101 aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc
     2161 ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct
     2221 accggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2281 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     2341 aatgagtgag gtaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2401 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2461 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2521 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2581 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2641 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2701 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2761 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2821 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2881 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2941 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3001 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3061 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     3121 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3181 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3241 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3301 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3361 gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg
     3421 ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa
     3481 aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc
     3541 agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct
     3601 gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag
     3661 ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca
     3721 tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg
     3781 aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggttcatttc gaaccccaga
     3841 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
     3901 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
     3961 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
     4021 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgtcat
     4081 gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg
     4141 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
     4201 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
     4261 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     4321 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     4381 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     4441 atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa
     4501 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     4561 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
     4621 gcaatccatc ttgttcaatc atactcttcc tttttcaata ttattgaagc atttatcagg
     4681 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4741 ttccgcgcac atttccccga aaagtgccac ctgaacgaag catctgtgct tcattttgta
     4801 gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     4861 acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt
     4921 ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     4981 tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt
     5041 cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga
     5101 ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt
     5161 aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag
     5221 cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag
     5281 ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga
     5341 aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt
     5401 gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta
     5461 tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa
     5521 atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat
     5581 agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg
     5641 gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg
     5701 cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa
     5761 taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca
     5821 acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg
     5881 tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat
     5941 atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc
     6001 catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac
     6061 tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat tggatcatat
     6121 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     6181 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     6241 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     6301 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     6361 gtgcaccata gatcaacgac attactatat atataatata ggaagcattt aatagaacag
     6421 catcgtaata tatgtgtact ttgcagttat gacgccagat ggcagtagtg gaagatattc
     6481 tttattgaaa aatagcttgt caccttacgt acaatcttga tccggagctt ttcttttttt
     6541 gccgattaag aattaattcg gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt
     6601 ggtgactatt gagcacgtga gtatacgtga ttaagcacac aaaggcagct tggagtatgt
     6661 ctgttattaa tttcacaggt agttctggtc cattggtgaa agtttgcggc ttgcagagca
     6721 cagaggccgc agaatgtgct ctagattccg atgctgactt gctgggtatt atatgtgtgc
     6781 ccaatagaaa gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag
     6841 catataaaaa tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta
     6901 aggaggatgt tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag
     6961 atgagtcgtg gcaagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat
     7021 ttccaaaaga ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc
     7081 ccttgtttga ttcagaagca ggtgggacag gtgaactttt ggattggaac tcgatttctg
     7141 actgggttgg aaggcaagag agccccgaaa gcttacattt tatgttagct ggtggactga
     7201 cgccagaaaa tgttggtgat gcgcttagat taaatggcgt tattggtgtt gatgtaagcg
     7261 gaggtgtgga gacaaatggt gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg
     7321 ctaagaaata ggttattact gagtagtatt tatttaagta ttgtttgtgc acttgccgat
     7381 ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
     7441 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     7501 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     7561 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     7621 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     7681 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     7741 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     7801 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     7861 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     7921 gaagggcgat
//
